I personally had Botox early in my first trimester before I knew I was pregnant, and everything was completely fine. Most likely Botox has no negative effects, but large studies are required to prove it, and no one will ever perform that study because the potential rewards (cosmetic) will never be enough to justify even a small potential amount of risk to a pregnancy. You can start using Botox (or fillers or latisse) again when you are done breast feeding. Happy wishes!!!